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read deep sequencing  (Azenta)

 
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    Azenta read deep sequencing
    Read Deep Sequencing, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/read deep sequencing/product/Azenta
    Average 86 stars, based on 1 article reviews
    read deep sequencing - by Bioz Stars, 2026-05
    86/100 stars

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    Illumina deep sequencing of RPgV at various points during mouse adaptation. The genome position of RPgV/maPgV is shown along the X-axis, with a schematic of predicted mature proteins shown in green across the top. The frequency of non-synonymous mutations (red) and synonymous variants >5% relative to the RPgV consensus sequence are shown along the left Y-axis, with a dashed black line denoting 50% frequency (i.e., consensus-level variants). Coverage is shown in gray on a log10 scale along the right Y-axis with a read-depth cutoff of 100 shown as a gray dashed line, below which variants were not called. The pooled “maPgV stock” described in is highlighted in yellow. Note that some samples were sequenced via unbiased deep sequencing and others were sequenced by multiplexed PCR amplicon sequencing, generating the “mountainous” versus “city-scape” appearing coverage plots, respectively.

    Journal: PLOS Pathogens

    Article Title: Determinants of pegivirus persistence, cross-species infection, and adaptation in the laboratory mouse

    doi: 10.1371/journal.ppat.1012436

    Figure Lengend Snippet: Illumina deep sequencing of RPgV at various points during mouse adaptation. The genome position of RPgV/maPgV is shown along the X-axis, with a schematic of predicted mature proteins shown in green across the top. The frequency of non-synonymous mutations (red) and synonymous variants >5% relative to the RPgV consensus sequence are shown along the left Y-axis, with a dashed black line denoting 50% frequency (i.e., consensus-level variants). Coverage is shown in gray on a log10 scale along the right Y-axis with a read-depth cutoff of 100 shown as a gray dashed line, below which variants were not called. The pooled “maPgV stock” described in is highlighted in yellow. Note that some samples were sequenced via unbiased deep sequencing and others were sequenced by multiplexed PCR amplicon sequencing, generating the “mountainous” versus “city-scape” appearing coverage plots, respectively.

    Article Snippet: Raw Illumina deep sequencing reads can be accessed in NCBI’s Short Read Archive (SRA) under accession numbers SAMN41664767–SAMN41664785 (BioProject ID PRJNA1119917).

    Techniques: Sequencing, Amplification

    Summary of all consensus-level variants detected in the expanded sequencing dataset. Equivalent analysis to that shown for a subset in can be found in . Non-synonymous and synonymous variants are shown in red and blue, respectively. Coverage > 100 reads is shown in gray. The thirteen mutations that consistently accumulate during adaptation to the murine host are in bold along the bottom.

    Journal: PLOS Pathogens

    Article Title: Determinants of pegivirus persistence, cross-species infection, and adaptation in the laboratory mouse

    doi: 10.1371/journal.ppat.1012436

    Figure Lengend Snippet: Summary of all consensus-level variants detected in the expanded sequencing dataset. Equivalent analysis to that shown for a subset in can be found in . Non-synonymous and synonymous variants are shown in red and blue, respectively. Coverage > 100 reads is shown in gray. The thirteen mutations that consistently accumulate during adaptation to the murine host are in bold along the bottom.

    Article Snippet: Raw Illumina deep sequencing reads can be accessed in NCBI’s Short Read Archive (SRA) under accession numbers SAMN41664767–SAMN41664785 (BioProject ID PRJNA1119917).

    Techniques: Sequencing